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Dissolved Oxygen

 

Protocols below are for use with Pasco probes. Click here for protocols to use the Vernier dissolved oxygen probe.

Materials:

PasPort Xplorer
PasPort dissolved oxygen sensor
PasPort dissolved oxygen probe
USB cable
DataStudio software
Temperature and Barometric pressure at your location
Deionized water wash bottle

Steps for calibrating the dissolved oxygen sensor:
1. Connect the dissolved oxygen probe to the dissolved oxygen sensor by screwing it in.
2. Connect the dissolved oxygen sensor to the Pasport Xplorer device.
3. Connect the PasPort Xplorer device to the computer with the USB cable.
4. Open the DataStudio software and select “Launch DataStudio.” (or "Create an Experiment")
5. Click on the “Setup” button located near the top of the screen.
6. Make sure the "mg/L" checkbox is checked in the Pasport setup window.
7. Click on the “Calibrate” button in the setup window.
8. Refer to the table 1 & table 2 below and enter the appropriate concentration (g/mL) of dissolved oxygen value depending on the pressure (in. Hg) and temperature (°C) at your location. (If this is unknown, the PasPort temperature and barometer can be used to take accurate measurements.)
9. Place the dissolved oxygen probe into the deionized water and click the “Set” button when the dissolved oxygen reading stabilizes.
10. Press the "Ok" button and the sensor should be calibrated.

Table 1: Concentration (mg/L) of Dissolved O2 at Saturation

 

29.5
in. Hg

29.7
in. Hg

29.9
in. Hg

30.1
in. Hg

30.3
in. Hg

30.5
in. Hg

30.7
in. Hg

30.9
in. Hg

31.1
in. Hg

31.3
in. Hg

0°C
14.37
14.47
14.57
14.66
14.76
14.86
14.95
15.05
15.15
15.24

1°C
13.98
14.08
14.17
14.27
14.36
14.45
14.55
14.64
14.73
14.83

2°C
13.61
13.70
13.79
13.88
13.97
14.07
14.16
14.25
14.34
14.43

3°C
13.25
13.34
13.43
13.52
13.61
13.69
13.78
13.87
13.96
14.05

4°C
12.90
12.99
13.08
13.16
13.25
13.34
13.42
13.51
13.60
13.68

5°C
12.57
12.66
12.74
12.83
12.91
13.00
13.08
13.16
13.25
13.33

6°C
12.25
12.34
12.42
12.50
12.58
12.67
12.75
12.83
12.91
13.00

7°C
11.95
12.03
12.11
12.19
12.27
12.35
12.43
12.51
12.59
12.67

8°C
11.66
11.74
11.81
11.89
11.97
12.05
12.13
12.21
12.29
12.36

9°C
11.38
11.45
11.53
11.61
11.68
11.76
11.84
11.91
11.99
12.07

10°C
11.11
11.18
11.26
11.33
11.41
11.48
11.56
11.63
11.71
11.78

11°C
10.85
10.92
10.99
11.07
11.14
11.21
11.29
11.36
11.43
11.51

12°C
10.60
10.67
10.74
10.81
10.89
10.96
11.03
11.10
11.17
11.24

13°C
10.36
10.43
10.50
10.57
10.64
10.71
10.78
10.85
10.92
10.99

14°C
10.13
10.20
10.27
10.34
10.41
10.48
10.54
10.61
10.68
10.75

15°C
9391
9.98
10.05
10.11
10.18
10.25
10.32
10.38
10.45
10.52

16°C
9.70
9.77
9.83
9.90
9.96
10.03
10.10
10.16
10.23
10.29

17°C
9.50
9.56
9.63
9.69
9.76
9.82
9.89
9.95
10.01
10.08

18°C
9.30
9.37
9.43
9.49
9.56
9.62
9.68
9.75
9.81
9.87

19°C
9.12
9.18
9.24
9.30
9.36
9.43
9.49
9.55
9.61
9.67

20°C
8.93
9.00
9.06
9.12
9.18
9.24
9.30
9.36
9.42
9.48

21°C
8.76
8.82
8.88
8.94
9.00
9.06
9.12
9.18
9.24
9.30

22°C
8.56
8.65
8.71
8.77
8.83
8.89
8.95
9.01
9.06
9.12

23°C
8.43
8.49
8.55
8.61
8.66
8.72
8.78
8.84
8.90
8.95

24°C
8.28
8.33
8.39
8.45
8.50
8.56
8.62
8.67
8.73
8.79

25°C
8.13
8.18
8.24
8.29
8.35
8.41
8.46
8.52
8.57
8.63

26°C
7.98
8.04
8.09
8.15
8.20
8.26
8.31
8.37
8.42
8.48

27°C
7.84
7.89
7.95
8.00
8.06
8.11
8.17
8.22
8.27
8.33

28°C
7.70
7.76
7.81
7.86
7.92
7.97
8.02
8.08
8.13
8.18

29°C
7.57
7.63
7.68
7.73
7.78
7.84
7.89
7.94
7.99
8.05

30°C
7.44
7.50
7.55
7.60
7.65
7.70
7.76
7.81
7.86
7.91

 

29.5
in. Hg

29.7
in. Hg

29.9
in. Hg

30.1
in. Hg

30.3
in. Hg

30.5
in. Hg

30.7
in. Hg

30.9
in. Hg

31.1
in. Hg

31.3
in. Hg
31°C
7.32
7.37
7.42
7.47
7.52
7.58
7.63
7.68
7.73
7.78
32°C
7.20
7.25
7.30
7.35
7.40
7.45
7.50
7.55
7.60
7.65
33°C
7.08
7.13
7.18
7.23
7.28
7.33
7.38
7.43
7.48
7.53
34°C
6.97
7.02
7.07
7.11
7.16
7.21
7.26
7.31
7.36
7.41
35°C
6.86
6.90
6.95
7.00
7.05
7.10
7.15
7.19
7.24
7.29
36°C
6.75
6.79
6.84
8.89
8.94
8.98
7.03
7.08
7.13
7.18
37°C
6.64
6.69
6.73
6.78
6.83
6.88
6.92
6.97
7.02
7.06
38°C
6.53
6.58
6.63
6.67
6.72
6.77
6.81
6.86
6.91
6.95
39°C
6.43
6.48
6.52
6.57
6.62
6.66
6.73
6.75
6.80
6.85
40°C
6.33
6.38
6.42
6.47
6.51
6.56
6.60
6.65
6.70
6.74
41°C
6.23
6.28
6.32
6.37
6.41
6.46
6.50
6.55
6.59
6.64
42°C
6.13
6.18
6.22
6.27
6.31
6.36
6.40
6.45
6.49
6.53
43°C
6.04
6.08
6.13
6.17
6.21
6.26
6.30
6.35
6.39
6.43
44°C
5.94
5.99
6.03
6.07
6.12
6.16
6.20
6.25
6.29
6.33
45°C
5.85
5.89
5.94
5.98
6.02
6.06
6.11
6.15
6.19
6.24
46°C
5.76
5.80
5.84
5.88
5.91
5.97
6.01
6.06
6.10
6.14
47°C
5.67
5.80
5.84
5.88
5.93
5.97
6.01
6.06
6.10
6.14
48°C
5.57
5.62
5.66
5.70
5.74
5.78
5.83
5.87
5.91
5.95
49°C
5.49
5.53
5.57
5.61
5.65
5.69
5.73
5.78
5.82
5.86
50°C
5.40
5.44
5.48
5.52
5.56
5.60
5.64
5.68
5.72
5.77

 

Table 2: Approximate Barometric Pressure at Different Elevations

Elevation
(feet)

Pressure
(mm Hg)

Elevation
(feet)

Pressure
(mm Hg)

Elevation
(feet)

Pressure
(mm Hg)

0

760

2000

708

4000

659

250

753

2250

702

4250

653

500

746

2500

695

4500

647

750

739

2750

689

4750

641

1000

733

3000

683

5000

635

1250

727

3250

677

5250

629

1500

720

3500

671

5500

624

1750

714

3750

665

5750

618
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